Size selection of spheroids for drug efficacy studies
CELLNETTA Application Case Studies
The information obtained from pharmacological studies serves an important basis in determining human administration in drug development, making it necessary to provide highly objective scientific data.
For this reason, it is important to efficiently obtain reliable information from pharmacological studies.
Here, we will introduce a case study of a spheroid drug efficacy study using CELLNETTA, conducted by Professor Shin Hasegawa of the Laboratory of Protein Function Analysis at the NagahamaInstitute of Bio-Science and Technology.(As of February 2026)
Implementation method
- (1)Culture RAS-transfected NIH3T3/EGFP cells in a 37°C incubator for 24 hours to generate spheroids of various sizes (Figure 1).
- (2)Apply hydrophilic treatment to the CELLNETTA. *
- (3)In the uniform-sized group, after passing the cell suspension through a 180-µm mesh CELLNETTA and again through a 100-µm mesh CELLNETTA, select spheroids trapped on the meshes, one at a time, and place them in a U-bottom plate (375 wells).In the untreated-size group, without using CELLNETTA, select spheroids one at a time and place them in a U-bottom plate (375 wells) (Figure 2).
- (4)For each group, administer 1, 3, 10, 20, and 100 nM of the proteasome inhibitor Bortezomib, and after 48 hours of incubation at 37°C, measure the ATP activity levels using an ATP quantification assay .
* For more information, please refer to the “Hydrophilic Treatment Manual“ in the CELLNETTA User Guide.
Results
In a comparison of the ATP activity rates of the uniform-sized group using CELLNETTA and untreated-size group, the error bar in the uniform-sized group is smaller than that in the untreated-size group. This indicates that reliable results can be obtained by conducting drug efficacy studies using spheroids whose sizes have been uniformed by CELLNETTA.
These results indicate that the use of CELLNETTA to equalize the size of spheroids can produce highly accurate drug efficacy study results.
Product used in this application note
| Pore size | Gamma Irradiation | Package Quantity | Product number (P/N) |
|---|---|---|---|
| 100 µm | Gamma Irradiated | 1 | MZM1B100B75GA |
| 5 | MZM1B100B75GB | ||
| 180 µm (custom made) | Gamma Irradiated | 1 | Please contact us. |
Notes
- ●This product is not a medical device.
- ●This product is a sample for evaluation purpose.
- ●Please do not ship out your completed product with the sample.
- ●We shall not be liable for any claims on the sample in case it is shipped out to the market.
CELLNETTA Details
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This product is single-use only.
- Compatible with 50 mL centrifuge tubes
- Individually packaged
- Made in Japan
- Gamma irradiated
| Size compatible with 50 mL centrifuge tubes | |||
|---|---|---|---|
| Pore Size （μｍ） | Package Quantity | Part Number | Application Examples |
| 5 | 1 | MZM1B005B50GA | Purification of culture medium from cell suspensions Removal of red blood cells from whole blood/PBMC |
| 5 | MZM1B005B50GB | ||
| 10 | 1 | MZM1B010B50GA | Purification of single cells |
| 5 | MZM1B010B50GB | ||
| 15 | 1 | MZM1B015B50GA | Fractionation of clusters and single cells |
| 5 | MZM1B015B50GB | ||
| 20 | 1 | MZM1B020B50GA | Fractionation of clusters and single cells |
| 5 | MZM1B020B50GB | ||
| 100 | 1 | MZM1B100B75GA | Spheroid size-based sorting for drug efficacy testing |
| 5 | MZM1B100B75GB | ||
| 200 | 1 | MZM1B200B75GA | Fractionation of microcarrier beads Spheroid size control |
| 5 | MZM1B200B75GB | ||
*We recommend selecting a pore size slightly smaller than the target cells. Please contact us for guidance on selecting the optimal pore size for your application.
*Flow behavior may vary depending on cell morphology, flow conditions, and other factors.