Purification of culture media from cell suspensions
CELLNETTA Application Case Studies
The purification of culture media from cell suspensions is commonly performed in cell-based experiments and in the manufacturing process of biopharmaceuticals. Here, we will introduce the method to purify the culture media from HL-60 cell suspensions.
Implementation method
- (1)Prepare a suspension of HL-60 cells.*1
- (2)Apply hydrophilic treatment to the CELLNETTA. *2
- (3)Place a 5-µm mesh CELLNETTA into a centrifuge tube (Figure 1, Operation 1).
- (4)Pour the suspension through CELLNETTA (Figure 1, Operation 2).
*1 Conditions for preparation of suspension
| Cell | Type | HL-60 |
|---|---|---|
| Size | 14 µm | |
| Cell count | 9.70× 105 cells | |
| Cell concentration | 1.94× 105 cells/mL | |
| Solvent | Type | RPMI medium |
| Volume | 5 mL |
Place into a centrifuge tube
Pour the suspension
*2 For more information, please refer to the “Hydrophilic Treatment Manual“ in the CELLNETTA User Guide.
Results
An image of the suspension after processing using CELLNETTA is shown in Figure 2.
After processing using CELLNETTA, the cells in the cell suspension are trapped on the mesh (Figure 2-A, B).
Observations of the cell suspension that passed through CELLNETTA indicate that all HL-60 cells
are trapped on the mesh, and that the culture media is purified from the cell suspension (Table 1).
These results indicate that it is possible to purify the culture media from a cell suspension by using CELLNETTA.
This simple cell removal method using CELLNETTA is expected to be useful in the purification process of culture media after cell culture, and in the medium exchange procedure during cell culture.
(A)Cells trapped on the CELLNETTA mesh (B)Suspension after processing using CELLNETTA
| Input solution | Suspension after processing using CELLNETTA | ||
|---|---|---|---|
| Cell | Cell count | 9.70× 105 cells | None observed |
| Cell concentration | 1.94× 105 cells/mL | None observed | |
| Solvent | Volume | 5 mL | 4.5 mL |
Product used in this application note
| Pore size | Gamma Irradiation | Package Quantity | Product number (P/N) |
|---|---|---|---|
| 5 µm | Gamma Irradiated | 1 | MZM1B005B50GA |
| 5 | MZM1B005B50GB |
Notes
- ●This product is not a medical device.
- ●This product is a sample for evaluation purpose.
- ●Please do not ship out your completed product with the sample.
- ●We shall not be liable for any claims on the sample in case it is shipped out to the market.
CELLNETTA Details
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This product is single-use only.
- Compatible with 50 mL centrifuge tubes
- Individually packaged
- Made in Japan
- Gamma irradiated
| Size compatible with 50 mL centrifuge tubes | |||
|---|---|---|---|
| Pore Size （μｍ） | Package Quantity | Part Number | Application Examples |
| 5 | 1 | MZM1B005B50GA | Purification of culture medium from cell suspensions Removal of red blood cells from whole blood/PBMC |
| 5 | MZM1B005B50GB | ||
| 10 | 1 | MZM1B010B50GA | Purification of single cells |
| 5 | MZM1B010B50GB | ||
| 15 | 1 | MZM1B015B50GA | Fractionation of clusters and single cells |
| 5 | MZM1B015B50GB | ||
| 20 | 1 | MZM1B020B50GA | Fractionation of clusters and single cells |
| 5 | MZM1B020B50GB | ||
| 100 | 1 | MZM1B100B75GA | Spheroid size-based sorting for drug efficacy testing |
| 5 | MZM1B100B75GB | ||
| 200 | 1 | MZM1B200B75GA | Fractionation of microcarrier beads Spheroid size control |
| 5 | MZM1B200B75GB | ||
*We recommend selecting a pore size slightly smaller than the target cells. Please contact us
for guidance on selecting the optimal pore size for your application.
*Flow behavior may vary depending on cell morphology, flow conditions, and other
factors.