User Guide and Case Studies

CELLNETTA User Guide

• Hydrophilic treatment manual

  For best results, please refer to the hydrophilic treatment manual before using CELLNETTA.

  Download PDF

CELLNETTA Case Studies

The following are reports on specific issues, protocol/method, and results.

• Application Note Vol.1Fractionation of cells and microcarrier beads

  This case study shows how to separate beads from a suspension containing microcarrier beads and cells.

  Keywords:

  • Removing microcarrier beads

  Download PDF

• Application Note Vol.2Fractionation of cell clusters and single cells
  - Application to CTC research -

  This case study shows the fractionation of single cell CTCs and cluster CTCs using CELLNETTA, as conducted by Mr. Manabu Maeshiro and associate Professor Satoru Shinriki at the Department of Molecular Laboratory Medicine, Faculty of Life Sciences, University of Kumamoto.

  Keywords:

  • CTC(Circulating tumor cells)
  • Single cell CTC
  • Cluster CTC
  • Fractionation of blood cells

  Download PDF

• Application Note Vol.3Equalization of spheroid size in drug efficacy studies

  This case study shows spheroid drug efficacy test using CELLNETTA as conducted by Professor Makoto Hasegawa at the Protein Function Analysis Laboratory, Nagahama Institute of Bio-Science and Technology.

  Keywords:

  • Spheroid
  • Drug efficacy research
  • Drug efficacy
  • Toxicity testing
  • Safety testing (alternative to animal testing)

  Download PDF

• Application Note Vol.4Single cell purification

  This case study shows purifying single cells from an aggregate cell suspension using CELLNETTA as conducted by Professor Masahiro Inoue at the Department of Clinical Bio-resource Research and Development, Graduate School of Medicine, Kyoto University.

  Keywords:

  • Single cell
  • Aggregate cell removal
  • Red blood cell removal

  Download PDF

• Application Note Vol.5Purification of culture media from cell suspensions

  This case study shows purification of a medium from a cell suspension.

  Keywords:

  • medium purification
  • Culture supernatant purification
  • Medium exchange

  Download PDF

• Application Note Vol.6 Monocyte removal for the fractionation of lymphocytes from PBMCs

  This case study shows monocyte removal in lymphocyte subsets from PBMC using CELLNETTA as conducted by Mr. Norihisa Mikami at the R&D Division of RegCell.

  Keywords:

  • Lymphocytes
  • Monocyte
  • PBMC(peripheral blood mononuclear cells)

  Download PDF

• Application Note Vol.7Removal of debris and red blood cells for preprocessing of single cell analysis

  This case study shows the removal of debris and red blood cells for prepossessing of single cell analysis as conducted by associate Professor Satoshi Ueha of the Division of Molecular Regeneration of Inflammatory and Immune Diseases (Matsushima Lab), Tokyo University of Science.。

  Keywords:

  • Single cell analysis
  • Single cell RNA-seq (scRNA-seq)

  Download PDF

CELLNETTA details